Please refer to the Obsolete Microplate Reader Models for more information. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax ABS. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. #655075 and #781075) SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax Injector Cartridge *Figure 1. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . All stand-alone instrument functions can be controlled using the software. Microplate readers can be either single-function instruments or “multimode. The contents of the SpectraDrop Microplate kit depend on kit type. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. #T-0254) • Lysozyme (Sigma cat. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Running both standard spectrophotometer and microplate. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. 69: 85. 19 H mm that meet microplate industry standards. Compare plate readersThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 03 pM. Omnia® Compatible Microplate Reader Documentation Version No. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Published Dec 16, 2020 | Updated Dec 06, 2021. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 3. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. 69: 85. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. #3610) BrightMax sealing films (Genesee cat. book Page 9 Wednesday, July 7, 2010 2:09 PM. LVF Monochromator™ : Excitation and Emission. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. microplate . Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 3. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. added to 96-well at 37 °C for 2 h, then the absorbance was measured at 450 nm by a. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. 984. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. No additional manuals are available. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Efficient cell washing with minimal cell loss and optimal assay performance. The reader adds up to three transfers of assay reagents from a 96- or 384. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Published Dec 16, 2020 | Updated Dec 16, 2020. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. This reader's dual monochromators for. SpectraDrop High-Throughput Screening (HTS) Kit. (Figure 1). Multi-Mode Microplate Reader for . It includes both shaking and incubation functions, and is compatible with 96- well microplates. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. 96- or 384-well microplate compatibility. Seller assumes all responsibility for this listing. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. M3-M5e_manual. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. 2. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. 98 VMax R2 = 0. The Perkin Elmer Wallac 1420. ©2010 molecular Devices, inc. #SpectraMax L Config) Methods. 96-well solid bottom, clear polystyrene microplates (Greiner cat. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 500 USD. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Bioz Stars score: 86/100, based on 1 PubMed citations. Learn more 0200-7008 6- to 1536-well plates. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 6:30 . No additional manuals are available. Detection by Low noise PMT with simultaneous digital and analog modes. 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. Plate Formats: 6 to 384-well microplates. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Use the provided 3. Use standard plate unless you see your type listed. This unlocks the transport slide. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Standard SBS 96 and 384 well plates (128. section, select plate section titled. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 375 pM. 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Supplementary Information. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . The SpectraMax® ABS Plus Microplate Reader can run . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 96-well: 0. #L6876) • Guanidine hydrochloride (Sigma cat. Fluorescence Microplate Reader Comparison Chart. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. flexibility of monochromator-based systems. 1. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. It features a modular design that allows users to add or change detection cartridges according to their needs. When reading at wavelengths. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. 0 OD Photometric resolution: 0. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. the iD5 reader’s filter system. Materials. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. Growth medium was then replaced with fatty acid uptake solution. Microplates . ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . Instrument settings are listed in Table 1. Fluorescence Microplate Reader Comparison Chart. Documentation:1. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. $489. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). In addition, the iD5 reader measures TRF and FP and. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Molecular Devices' microplate readers. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. We would like to show you a description here but the site won’t allow us. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. g. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. 92: 88. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. best-in-class data . 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). the appropriate well of the microplate. 2 mm x 86 mm) Height: between 13. /well integration in 384-well luminescence mode Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Blank wells containing 50 µL culture medium only (n = 8) were also prepared. 030 fmol Fluorescence Polarization (FP). Vrla used the device to quantify the concentration of a small molecule produced by. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Step 1: Turn on your microplate reader. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Paradigm: Delta mP. Average StDev (mP) 1. The system provides options for 20-, 40-,. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Related Products: Low Binding Plates. F3/F5 Multi-Mode Microplate Readers. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. 1 OD at 230 nm. Transfer of an assay to 96-well microplate technology increases throughput significantly. explore more. This reader also makes use of a unique temperature-independent methodology for path length correction. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 125 pM. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Microscopy. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Built in near field communication (NFC). Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. Wide range of applications The VersaMax reader covers. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Related Products: Low Binding Plates. • 0. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. explore more. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Making it possible to perform kinetic analysis of solid. the assay. Read More. Products : General, FilterMax F5 reader. OEM Molecular Devices All Microplate Reader, Top r. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. It also integrates with the. 2600A October 2023. 2022 Harini Natarajan et al. Plate reader instructions Computer. Other technologies such as imaging. flat-bottom: 50-60 µl. Multimode Reader Comparison Chart. #L6876) Guanidine hydrochloride (Sigma cat. Plate Formats: 6 to 384-well microplates. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. Four wash/rinse bottles are included and configurable for both 96- and 384. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Retrieving. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. The CellTiter-Glo assay from Promega uses luciferase. The validation kit included individual tubes of diluted fluorophores:. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 96-well microplate at 200 µL/well. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. A. We would like to show you a description here but the site won’t allow us. Microplate luminescent reader assay for ERK activity. Use black microplates. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. i3x. Description. Table 1: Categories of heating element design. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Place the microplate in the drawer. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Three kits covering a wide range of dsDNA concentrations. Materials. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. absorbance, fluorescence, and luminescence with the added functionality of. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. 03 pM 384-well: 0. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. book Page 1 Wednesday, October 12, 2011 4:56 PMBuilt around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). > Molecular Devices LLC > microplate reader. 340‚ 850 nm. microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . Eight filter modes enable applications such as protein quantification, cell viability and ELISA. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Compare specifications and request a quote from leading suppliers. M5. The system provides automation for up to 50 microplates for easy walkaway automation. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. (FP) configured microplate readers. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. ©2017 Molecular Devices, LLC . #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. . 96, 384, 24, 12, 6 well types. Microplate is validated by molecular devices for the FLIPR reader. Align the two slots on the bottle holder with the two pegs on the rail. Microplate Spectrophotometers. for ValitaTiter Assays. Optimized for fluorescence microplate readers. An industry-renowned instrument for monitoring GPCRs and ion channels. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. (96, 384, or 1536 wells) and the "Select Specific". The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. With various on-board features and. Ordering information FlexStation 3 Microplate Reader Part Number: FLEX3 • FlexStation 3 Base System Consumables 96-Well, FlexStation Pipet Tips (Black) Part Number: 9000-0911 • 200 µL capacity • Includes 3 vials each of FLIPR Calcium 6 and 6-QF dyes Reagents FLIPR® Calcium 6 Assay Evaluation Kit Part Number. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Multimode Readers Comparison Chart. For more sample throughput, the system can be. A control well was set up to verify. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. 0 H x 60. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Detergent Compatible Bradford assay. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. #M5e) Methods Microplate reader compatibility test. Integrated stacker option. 8. of 0. In simplest terms, a plate reader measures and. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. 384 Awareness Technology ChroMate BioTek Synergy Mx. Avoid losing high signal. 05. Whatever your needs, you’ll find a microplate reader that can meet them. Endpoint, kinetic, spectrum and area-well scanning Click here. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan molecular devices Japan, osaka. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 125 pM * Specifications listed are for europium 96-well: 0. Add 150 µL reagent to each well. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. accurately over a wide dynamic range. Reading Capabilities: cuvette or microplate. ScanLater Western Blot Detection System. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. For biochemical. 384-well: 0. Microplate Reader, Molecular Devices Optimax, Refurbished. # 651101) the iD5 reader’s filter system. Learn more about how absorbance is measured, and some key applications that utilize. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Photometric and kinetic analysis mode. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. In addition, the iD5 reader measures TRF and FP and. It measures the optical density (OD) of samples in both 96- and 384-well. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. #N207) LAL Reagent Water (Lonza cat. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Well scanning With well scanning, multiple points within each well can be read, providing a high. Listing: #4772697. Microplate Reader (Molecular Devices cat. The system provides automation for up to 50 microplates for easy walkaway automation. SpectraMax iD5. microplates or in a cuvette at a selected. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). 96-well microplate; Immunoassay analyzer;VersaMax price. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. Figure 2. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Setup Guide. 384 well polystyrene microplates have plate dimensions of 127. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. 39. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Also refer to this Multimode Reader Comparison Chart. Lucid Scientific. 76 L x 85. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. robotics-automation-compatible. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Description.